@AFC_cristal
| Intitulé du poste : | Postdoc position |
| Ville : | Bordeaux |
| Laboratoire/Institut : | The candidate will work in the PRISM team (Protein-RNA Interactions by Structural Methods) at the University of Bordeaux (France). PRISM is one of the six teams that compose the ARNA laboratory (Nucleic Acids: Natural and Artificial Regulations): https://arna.cnrs.fr/en/home-page/. Our laboratory is located in the new Bordeaux Biologie Santé building (BBS) and have nearby other institutes for research such as the Institut Européen de Chimie et Biologie. IECB houses a cryo electron microscope (Talos Arctica), for improved resolution we will apply to the French Infrastructure for Integrated Structural Biology (FRISBI) instruments such as the Titan Krios, the latest and most powerful instrument. In addition, IECB houses Solution state NMR instruments including an 800 (cryogenic probe), 700, and 400 MHz spectrometers. |
| Description du poste : | The candidate will work in a project at the interface between NMR and cryo-EM. With general objectives centered on structure determination of DNA G-quadruplexes in complex with regulatory proteins. The project is directed by Gilmar Salgado (Assistant Professor at U. Bordeaux). The position is funded by the French Institut national de la santé et de la recherche médicale (Inserm). Stretches of DNA on the promoter region of many genes, including those known implicated in diseases such as cancer, Alzheimer, amyotrophic lateral sclerosis among others, are enriched with guanine-rich sequences that are the main target of regulatory proteins. Importantly, these sequences can fold into 3D structures via Hoogsteen type hydrogen-bonding where four guanines self-assembled into square planar arrangement, resulting in the formation of unusual DNA structures known as G-quadruplexes (G4s). Those unusual structures are the target of many regulatory proteins and are perceived as modulators for gene transcription. We want to study those protein-DNA complexes in order to decipher their regulatory roles in gene expression. Our main goal is to simultaneously combine information from multiple sources (cryo- EM, NMR, smFRET, X-ray & SAXS) applying modern Integrative Structure Biology (ISB) approaches, and form a global view of the DNA-Protein complex system and their mode of gene regulation. The project is a collaboration between 3 French laboratories and one from Italy. |
| Compétences requises : | Minimum Qualifications Required: - Ph.D. in Biophysics/Biochemistry or related fields with specialization in structural biology methods (preference for liquid state NMR) - Comfortable using a variety of biophysical tools such as ITC, CD among others. - Good written and oral communication skills - Experience in prokaryotic recombinant protein production - Experience in launching 2D/3D NMR spectra and structure calculations - Able to work in an autonomous and organized manner - Strong leading role and management skills |
| Procédure pour candidater: | The position will start as soon as November 2024. The position is a full-time, 24-month appointment. The salary range is listed according to Inserm for experienced postdoctoral fellow’s rules and depends on the experience and annuities after the thesis. Please send a cover letter together with a curriculum vitae and contact information for all past |
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