Intitulé du poste : Post-doctorant in membrane structural biology
Ville : Marseille
Laboratoire/Institut : UMR7256 Information Génomique et Structurale CNRS-AMU
Description du poste : Structural and functional characterization of the machinery used by the Mimiviridae to compact their genomes 

The IGS laboratory is offering two Post-doctoral positions and to PhD thesis grants in the context of and ERC grant. The present position will aim to elucidate how the Mimiviridae genome is packaged inside the icosahedral capsids. As the disorganized DNA is packaged through at least one lipidic membrane into a rod-shaped helicoidal structure with a 30nm section, a specific assembly machinery must be devoted to the genome packaging. The ultimate goal of the project will be to compare this machinery with the DNA translocation machineries used in the cellular and the viral world.

The first aim of the project will be to extract the membrane from the capsids to identify the membrane anchored proteins (viral and cellular). Mimivirus genome encodes a DNA packaging ATPase that was proposed to be involved in genome packaging. It is one of the few core genes conserved in all Nucleocytoplasmic large DNA viruses. Moreover, this ATPase is a closer homolog to prokaryotic chromosome segregation motors than to other viral packaging motors, such as large terminase. The Packaging ATPase will thus be studied alone and will be used as a bait to identify its possible partners, in vitro and in infected cells. Together with a PhD student, the candidate will then express the individual proteins and will use standard methodologies including the use of nanodiscs, crystallization and single particle Cryo-EM to retrieve structural information of individual proteins and complexes. In vitro reconstitutions of complexes will also be performed. To apply for the Position at IGS, the candidate must deposit his/her CV with a complete list of publications, a motivation letter and at least one reference letter at
Durée du contrat : Between 1 and 4 years
Date de prise de fonction : 01-10-2019
Personne à contacter : Chantal Abergel
Téléphone : 0491825422
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